Label-fracture: a method for high resolution labeling of cell surfaces.
نویسندگان
چکیده
منابع مشابه
Label-fracture: a method for high resolution labeling of cell surfaces
We introduce here a technique, "label-fracture," that allows the observation of the distribution of a cytochemical label on a cell surface. Cell surfaces labeled with an electron-dense marker (colloidal gold) are freeze-fractured and the fracture faces are replicated by plantinum/carbon evaporation. The exoplasmic halves of the membrane, apparently stabilized by the deposition of the Pt/C repli...
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We introduce fracture-flip, a method of obtaining high-resolution views of membrane surfaces. The method, a corollary of label-fracture, is based on the stabilization of the exoplasmic halves of membranes by carbon evaporation. Inversion of these casts followed by Pt/C imaging leads to new views of the cell surface at macromolecular resolution.
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1984
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.99.3.1156